12592
Metal Packed GC Column (General Configuration)
phase 3% SP®-1500, matrix 80/120 Carbopack B support, L × O.D. × I.D. 10.0 ft (3.0 m) × 1/8 in. × 2.1 mm
material
stainless steel column
Quality Level
parameter
0-230 °C temperature (isothermal or programmed)
technique(s)
gas chromatography (GC): suitable
L × O.D. × I.D.
10.0 ft (3.0 m) × 1/8 in. × 2.1 mm
matrix
80/120 Carbopack B support
matrix active group
3% SP®-1500 phase
column type
packed GC
compatibility
for use with General Configuration
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General description
An economical metal column should be used for less demanding applications where the inertness of glass is not required. This column is of a general configuration, and can be carefully bent to fit most instruments.
Packed column made up of stainless steel are basically employed when higher carrier gas flow rates are employed as they can sustain elevated pressure. Stainless steel columns are usually washed with dilute hydrochloric acid, then with water followed by methanol, acetone, methylene dichloride and n-hexane in order to remove any corrosion products and traces of lubricating agents.
Application
Carbopack B SP-1500 packed gas chromatographic column maybe used for the analysis of toluene in order to study the activity and three dimensional distribution of toluene degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy.
Legal Information
SP is a registered trademark of Sigma-Aldrich Co. LLC
Storage Class
11 - Combustible Solids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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S Møller et al.
Applied and environmental microbiology, 62(12), 4632-4640 (1996-12-01)
As a representative member of the toluene-degrading population in a biofilter for waste gas treatment, Pseudomonas putida was investigated with a 16S rRNA targeting probe. The three-dimensional distribution of P. putida was visualized in the biofilm matrix by scanning confocal
Introduction to Analytical Gas Chromatography.
Scott RPW.
Science, 102-108 (1997)
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