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TRUE4

TrueGel3D Hydrogel Kits

FAST-PVA, CD cell-degradable crosslinker

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
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shipped in

dry ice

storage temp.

−70°C

General description

The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

Application

TRUE4-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been preferred to use PVA-based gels over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes.

Features and Benefits

  • Fast gelling (takes few seconds to minutes after addition of crosslinker)
  • Flexibility in modulating gel stiffness
  • Contains MMP-cleavable crosslinker
  • Cells cannot be recovered
  • Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

Other Notes

TRUE4-1KT contains FAST-PVA polymer solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Disclaimer

Avoid prolong exposure of CD cell-degradable crosslinker to air and store FAST-PVA in small portions to avoid frequent freeze-thaw cycles.

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pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

10 - Combustible liquids

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Protocols

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.

Articles

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

View All Articles

Global Trade Item Number

SKUGTIN
TRUE4-1KT04061835539277