Skip to Content
MilliporeSigma

Key Documents

View All Documentation

TP0300

Total Protein Kit, Micro Lowry, Peterson′s Modification

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

sufficient for ~200 assays

Quality Level

200

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Biochem/physiol Actions

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
116K6818

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

Product Information Sheet - TP0300
Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Cuantificación de proteínas

Métodos de cuantificación de proteínas, reactivos y tecnología de inmunoanálisis para la medición precisa de las concentraciones de proteínas en una variedad de muestras.

View All Related Content
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive
Dalia A Elgamal et al.
Molecular and cellular endocrinology, 503, 110700-110700 (2020-01-07)
Genetic and epigenetic factors contribute equally to the pathogenesis of type 1 diabetes mellitus. Sodium butyrate (NaB) has been reported to improve glucose homeostasis by modulation of the p38/ERK MAPK pathway. This work aims to evaluate the effect of NaB
Expression of alpha-gustducin and alpha-transducin, G proteins coupled with taste receptors, in boar sperm
Spinaci M, et al.
Theriogenology, 82(1), 144-151 (2014)
View All Related Papers

Global Trade Item Number

SKUGTIN
TP0300-1KT04061835574100