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T6634

Thrombin from bovine plasma

Name: Thrombin from bovine plasma
Brand: SIGMA

lyophilized powder, 600-2,000 NIH units/mg protein (biuret)

Synonym(s):

Factor IIa

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About This Item

CAS Number:

9002-04-4

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-648-7

MDL number:

MFCD00166053

EC Number:

3.4.21.5 (BRENDA, IUBMB)

Specific activity:

600-2,000 NIH units/mg protein (biuret)

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Properties

form

lyophilized powder

Quality Level

300

specific activity

600-2,000 NIH units/mg protein (biuret)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

UniProt accession no.

P00735

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... F2(280685)

Description

General description

Thrombin is a sodium-activated type II enzyme. It contains two anion binding exosites, ABE-I and ABE-II. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond. The B chain of a-thrombin includes a carbohydrate portion.

Application

Thrombin from bovine plasma has been used to study its effect on the perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation, and migration in culture. It has also been used in fibrin degradation assay to measure nattokinase activity.

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess global haemostasis and point of care testing.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Thrombin is a proteolytic enzyme critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. Thrombin promotes platelet aggregation. Therefore, thrombin is the final coagulation protease in hemostasis, promoting both procoagulant and anticoagulant effects. It is used to treat bleeding from capillaries and small venules.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Global haemostasis and point of care testing.

Y Dargaud et al.

Haemophilia : the official journal of the World Federation of Hemophilia, 18 Suppl 4, 81-88 (2012-06-29)

The evaluation of the coagulation profile has used so far either clotting-based or chromogenic assays with different endpoints. Clotting-based techniques are the most used worldwide, and they certainly are useful for diagnosis of clotting factor deficiencies. However, the information provided

Thrombin as an Agent

xPharm: The Comprehensive Pharmacology Reference null

Thrombin as a Target