T6567
Trypsin Protease
Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas
Synonym(s):
Porcine Trypsin, Trypsin for Mass Spectropetry
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About This Item
Product Name
Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated
biological source
Porcine pancreas
Quality Level
product line
BioReagent
solubility
1 mM HCl: soluble 1 mg/mL, clear, colorless
shipped in
wet ice
storage temp.
2-8°C
General description
Application
- In-gel protein digestion and MALDI-TOF mass spectrometry analysis
- Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
- Surface proteome profiling of L. plantarum
- Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
- Mass spectrometry
Biochem/physiol Actions
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocols
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
Articles
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T6567-1MG | 04061835491971 |
| T6567-20UG | 04061835519132 |
| T6567-5X20UG | 04061835575015 |