SRP3215
JE/MCP-1 (CCL2) from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CCL2, JE, MCAF
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
13.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... CCL2(20296)
General description
The monocyte chemoattractant proteins (MCPs) belong to the C-C chemokine family, and signal through C-C motif chemokine receptor-2 (CCR2) and, with the exception of MCP-1 or chemokine (C-C motif) ligand 2 (CCL2) , other CCR receptors. The MCP family cross reacts across species. The gene encoding CCL2 is localized on mouse chromosome 11. Recombinant murine JE is a 13.8kDa protein containing 125 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Biochem/physiol Actions
The monocyte chemoattractant proteins (MCPs) chemo attract and activate monocytes, activated T cells, basophils, natural killer (NK) cells and immature dendritic cells. It has a role in the survival and proliferation of microglia. Chemokine (C-C motif) ligand 2 (CCL2) has been shown to activate GTPases like cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate 1 (Rac1).
Physical form
Lyophilized with no additives.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Evidence for a cluster of genes on chromosome 17q11-q21 controlling susceptibility to tuberculosis and leprosy in Brazilians.
Jamieson SE
Genes and Immunity, 5(1), 46-57 (2004)
Vascular Stem/Progenitor Cell Migration Induced by Smooth Muscle Cell-Derived Chemokine (C-C Motif) Ligand 2 and Chemokine (C-X-C motif) Ligand 1 Contributes to Neointima Formation.
Yu B
Stem Cells, 34(9), 2368-2380 (2016)
Rollins, R.J., et al.
Cytokine Reference, 1145-1160 (2001)
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