SRP3162
sTNF-receptor Type I human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Soluble Tumor Necrosis Factor type I, TNF-R55, TNFAR, TNFRSF1A
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
0.25 ng/mL ED50
mol wt
18.3 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TNFRSF1A(7132)
General description
TNFR1 (tumor necrosis factor receptor 1) gene is localized to human chromosome 12p13, and encodes a 55kDa receptor showing a wide range of tissue expression. This receptor belongs to the family of receptors which contain repeating cysteine-rich extracellular motifs. TNFR1 contains a protein-protein interacting domain called death domain containing ∼70 amino acids. It also contains a silencer of death domains (SODD), a 60kDa domain, which inhibits ligand-independent self-association of TNFR1. TNFR1 is a member of the TNFR family, which are type I transmembrane proteins.
Recombinant human sTNFR1 is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
Recombinant human sTNFR1 is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
Biochem/physiol Actions
TNFR1 (tumor necrosis factor receptor 1) is involved in inflammatory responses, and the cleavage of the membrane form leads to production of soluble TNFR1. The soluble form competes with the membrane-bound form, and this leads to a reduction in the severity of the inflammatory responses. Mutations in this gene resulting in the disruption of conserved extracellular disulfide bonds, leads to lower levels of soluble TNFR1 forms, which is linked with autosomal dominant periodic fever syndromes, characterized by unexplained recurrent fevers and severe localized inflammation. Studies in two independent community-based elderly cohorts show that sTNFR1 is linked with increased mortality in cardiovascular and cancer cases.
Physical form
Lyophilized from 10 mM Tris, pH 8.0 + 20 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Germline mutations in the extracellular domains of the 55 kDa TNF receptor, TNFR1, define a family of dominantly inherited autoinflammatory syndromes.
McDermott MF et al
Cell, 97(1), 133-144 (1999)
Signalling pathways of the TNF superfamily: a double-edged sword.
Aggarwal BB
Nature Reviews: Immunology, 3(9), 745-756 (2003)
Soluble tumor necrosis factor receptor 1 (sTNFR1) is associated with increased total mortality due to cancer and cardiovascular causes - findings from two community based cohorts of elderly.
Carlsson AC et al
Atherosclerosis, 237(1), 236-242 (2014)
Aggarwal, B.B., et al.
Cytokine Reference, 1619-1632 (2001)
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