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SRE0026

Phosphatase substrate

Suitable for manufacturing of diagnostic kits and reagents

Synonym(s):

Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
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Quality Level

assay

≥97% (HPLC)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Hawa G, et al.
Analytical biochemistry, 549, 39-44 (2018)
Christina Papista et al.
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Masaya Yokota et al.
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not



Global Trade Item Number

SKUGTIN
SRE0026-1G04061826658611
SRE0026-25G04061833493472
SRE0026-10G04061833343456
SRE0026-50G04061833413128
SRE0026-5G04061833343463