Skip to Content
MilliporeSigma

SAB6012220

Anti-phospho-Raf1 (Ser259) Antibody, clone 23GB2645, Rabbit Monoclonal

newenhanced validation

clone 23GB2645, recombinant rabbit monoclonal, expressed in HEK293 cells

Synonym(s):

CMD1NN, CRAF, RAF, Raf-1

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit (Monoclonal)

Quality Level

100

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

23GB2645, recombinant monoclonal

form

buffered aqueous glycerol solution

mol wt

73 kDa

species reactivity

human, rat

packaging

vial of 1.5 mL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

1.2 mg/ml

technique(s)

flow cytometry: 1:2000
immunofluorescence: 1:100-:1000
western blot: 1:1,000-1:5,000

isotype

IgG

Ensembl | human accession no.

ENSG00000132155

NCBI accession no.

NP_002871.1

UniProt accession no.

P04049

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAF1(5894)

General description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Immunogen

A synthesized peptide corresponding to a sequence within amino acids 252-266 of human Phospho-Raf1 (S259) [NP_002871.1].

Physical form

Solution in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Other Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Not finding the right product?  

Try our Product Selector Tool.

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service