SAB4200710
Anti-Calponin antibody, Mouse monoclonal
clone hCP, purified from hybridoma cell culture
Synonym(s):
Anti-CNN-1, Anti-CNN-2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
conjugate
unconjugated
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
hCP, monoclonal
form
buffered aqueous solution
mol wt
antigen ~34 kDa
species reactivity
bovine, monkey, human, rabbit, ferret, mouse, hamster, rat, canine, pig
concentration
~1 mg/mL
technique(s)
immunoblotting: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human tonsil sections
immunoprecipitation (IP): suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CNN1(1264)
General description
Anti-Calponin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hCP hybridoma (also cites as CALP), produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Calponin (CNN) exists in three isoforms CNN1, CNN2 and CNN3. CNN2 gene is mapped to human chromosome 19p13.3. All three isoforms are expressed in smooth muscle. While CNN1 expression is confined only to smooth muscle, CNN2 and CNN3 are expressed in other cells including the neuronal tissue, fibroblast, myoblast and B lymphocyte. The three isoforms differ in the C-terminal region and comprise conserved actin-binding middle domain and a N-terminal calponin homology (CH) domain.
Immunogen
human uterus smooth muscle extract
Application
Anti-Calponin antibody, Mouse monoclonal may be used in:
- immunoblotting
- immunoprecipitation
- immunohistochemistry
Biochem/physiol Actions
Calponin is an F-actin, calmodulin, and tropomyosin binding protein that presents in both the cytoskeleton and contractile machinery of smooth muscle (SM) cells. The interaction of Calponin with actin is shown to inhibit myosin Mg-ATPase activity. The binding strength of calponin to tropomyosin is weakened in the presence of Ca2+. Calponin is involved in a variety of cellular processes including smooth muscle contraction, cell movement, shape change, angiogenesis, exocytosis and endocytosis. Monoclonal anti-Calponin can be used for the identification and localization of calponin in SM elements. Calponin levels may serve as a prognostic factor in hepatocellular carcinoma, renal cell carcinoma, osteosarcoma and melanoma.
Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Cytoskeletal targeting of calponin in differentiated, contractile smooth muscle cells of the ferret.
C A Parker et al.
The Journal of physiology, 508 ( Pt 1), 187-198 (1998-06-06)
1. Biochemical and quantitative image analysis methods were used to investigate the anatomical basis for the previously described agonist-induced redistribution of calponin. 2. At 140 nm resolution, the quantitative distribution of calponin in resting cells was statistically indistinguishable from that
Y J Mao et al.
The Journal of international medical research, 38(2), 711-719 (2010-06-03)
A case report of laryngeal myoepithelial carcinoma (MEC) is presented and the literature concerning prognostic factors in MEC is reviewed. A 61-year old man was admitted to hospital with hoarseness and progressive dyspnoea. On examination, both vocal cords were fixed
Calcium-dependent regulation of smooth muscle calponin by S100
Fujii T, et al.
Journal of Biochemistry, 116(1), 121-127 (1994)
Yuri Hojo et al.
The Journal of veterinary medical science, 74(5), 625-628 (2011-12-14)
A subcutaneous tumor in the left inguinal region was present in an 11-year-old female bloodhound. Histopathologically, the tumor showed invasive growth and extensive necroses, and it was composed of spindle-shaped, elongated, and stellate neoplastic cells accompanied by occasional giant cells
Christian W Kreipke et al.
Journal of cellular and molecular medicine, 13(2), 262-269 (2009-03-13)
Calponin (Cp) is an actin-binding protein first characterized in chicken gizzard smooth muscle (SM). This review discusses the role of Cp in mediating SM contraction, the biochemical process by which Cp facilitates SM contraction and the function of Cp in
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