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MilliporeSigma

SAB1409296

Sigma-Aldrich

Monoclonal Anti-SMAD5 antibody produced in mouse

clone 5E12, purified immunoglobulin, buffered aqueous solution

Synonym(s):

DKFZp781C1895, DKFZp781O1323, Dwfc, JV5-1, MADH5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5E12, monoclonal

form

buffered aqueous solution

mol wt

antigen 34.21 kDa

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

NCBI accession no.

BC009682

UniProt accession no.

Q99717

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMAD5(4090)

Immunogen

SMAD5 (AAH09682, 105 a.a. ~ 182 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
KPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFPDSFHQPNN

Physical form

Solution in phosphate buffered saline, pH 7.4

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
07264-5E12

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Jonathan R Peterson et al.
Science translational medicine, 6(255), 255ra132-255ra132 (2014-09-26)
Heterotopic ossification (HO) is the pathologic development of ectopic bone in soft tissues because of a local or systemic inflammatory insult, such as burn injury or trauma. In HO, mesenchymal stem cells (MSCs) are inappropriately activated to undergo osteogenic differentiation.

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