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S5171

Human Fibronectin

Name: Human Fibronectin
Brand: SIGMA

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Synonym(s):

sFN

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.75

MDL number:

MFCD00284706

Biological source:

human

Form:

solution

Technique(s):

cell culture | mammalian: suitable

Mol wt:

~235-270 kDa

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Properties

Product Name

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

product line

BioReagent

form

solution

mol wt

~235-270 kDa

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1 μg/cm²

UniProt accession no.

P02751

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

Description

Application

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Preparation Note

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Other Notes

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Disclaimer

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Articles

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Global Trade Item Number

SKU	GTIN
S5171-.5MG	04061836944766