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S1452

MISSION® siRNA Transfection Reagent

Synonym(s):

MISSION® siRNA Transfection Reagent

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106502
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product line

MISSION®

Quality Level

200

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C

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Articles

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Expresión y silenciamiento génicos

Sigma-Aldrich® Advanced Genomics es el principal proveedor de tecnologías de edición y silenciamiento de genes, entre ellas, CRISPR, Cas9, ARN guía sintético (sgRNA) y nucleasa con dedos de zinc (ZFN).

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Global Trade Item Number

SKUGTIN
S1452-100UL04061837743672
S1452-1ML04061836910990