RAB0448
Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines
96 assays
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.84
usage
96 assays
species reactivity
mouse, human
technique(s)
ELISA: suitable
capture ELISA: suitable
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... STAT4(6775)
mouse ... STAT4(20849)
Related Categories
General description
The Cell-Based Stat4 (pTyr693) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat4 (pTyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines.
Immunogen
Stat4 (pTyr693) synthetic phosphopeptide
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Kit Components Also Available Separately
Product No.
Description
SDS
- RABBLOCK5x Blocking Solution (Item F)SDS
- RABFIX1Fixing Solution (Item D)SDS
- RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
- RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
- RABPLATE1Uncoated MicroplateSDS
- RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
- RABSTOP1Stop SolutionSDS
See All (10)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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C M Bacon et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7307-7311 (1995-08-01)
Interleukin 12 (IL-12) is an important immunoregulatory cytokine whose receptor is a member of the hematopoietin receptor superfamily. We have recently demonstrated that stimulation of human T and natural killer cells with IL-12 induces tyrosine phosphorylation of the Janus family
K Yamamoto et al.
Molecular and cellular biology, 14(7), 4342-4349 (1994-07-01)
Interferon regulation of gene expression is dependent on the tyrosine phosphorylation and activation of the DNA-binding activity of two related proteins of 91 kDa (STAT1) and/or 113 kDa (STAT2). Recent studies have suggested that these proteins are substrates of Janus
Hua-Chen Chang et al.
The Journal of biological chemistry, 278(34), 32471-32477 (2003-06-14)
STAT4 (signal transducer and activator of transcription-4) mediates biological effects in response to interleukin-12 (IL-12). STAT4 has multiple domains that have distinct functions in signaling and gene activation. To characterize the role of the STAT4 N-terminal domain in mediating STAT4
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