R6750
Ribonucleic acid from baker′s yeast (S. cerevisiae)
Synonym(s):
RNA
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About This Item
Form:
powder
Solubility:
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
Storage temp.:
−20°C
Quality Level
form
powder
solubility
water: 10 mg/mL, clear to very slightly hazy (faint yellow to light yellow)
suitability
suitable for (suitable for use as a substrate for Rnase)
storage temp.
−20°C
General description
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is the substrate for RNase enzyme. The isolation of RNA from yeast is a complicated process and involves heating and freezing cycles of cells in the presence of phenyl and detergents (SDS).
Application
Ribonucleic Acid (RNA) from baker’s yeast (S. cerevisiae) is suitable for applications such as northern blot hybridization, reverse transcriptase-polymerase chain reaction (RTPCR), and cDNA construction.
Preparation Note
Prepared by a modification of Crestfield, A.M., et al., J. Biol. Chem., 216, 185 (1955).
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Lukas Bossaller et al.
Journal of immunology (Baltimore, Md. : 1950), 197(4), 1044-1053 (2016-06-30)
Systemic lupus erythematosus (SLE) is a chronic, life-threatening autoimmune disorder, leading to multiple organ pathologies and kidney destruction. Analyses of numerous murine models of spontaneous SLE have revealed a critical role for endosomal TLRs in the production of autoantibodies and
Hidekazu Iioka et al.
Nucleic acids research, 39(8), e53-e53 (2011-02-09)
The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins.
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R6750-100MG | 04061836695934 |
| R6750-1G | 04061836695941 |
| R6750-250MG | 04061833290026 |
| R6750-500MG | 04061833290033 |