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R4526

Reference Dye for Quantitative PCR

Name: Reference Dye for Quantitative PCR
Brand: SIGMA

100 ×, solution

Synonym(s):

Reference dye for qPCR, ROX

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About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

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Properties

form

solution

Quality Level

300

usage

sufficient for ≥600 reactions

feature

PCR Additives

concentration

100 ×

technique(s)

PCR: suitable

color

red

input

crude DNA

solubility

water: soluble

storage temp.

2-8°C

Description

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:

in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H410

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

ppe

Eyeshields, Gloves

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Protocols

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Articles

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Global Trade Item Number

SKU	GTIN
R4526-.3ML	04061836695354
R4526-20RXN	04061832933283
R4526-5ML	04061837693939