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P4744

Phosphatase substrate

Name: Phosphatase substrate
Brand: SIGMA

chromogenic, powder

Synonym(s):

4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24898495

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

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Properties

Product Name

Phosphatase substrate, powder

Quality Level

200

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

Application

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP).

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Global Trade Item Number

SKU	GTIN
P4744-25G	04061835516261
P4744-50G	04061834375166
P4744-5G	04061835522026
P4744-10G	04061835558353
P4744-1G	04061835558360