P3889
Polygalacturonic acid
≥85% (titration), from oranges
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About This Item
Linear Formula:
(C6H8O6)n
CAS Number:
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
biological source
oranges
Quality Level
assay
≥85% (titration)
form
powder
color
white to brown
mp
60 °C
solubility
aqueous NaOH: 1%, clear to hazy
storage temp.
room temp
SMILES string
O1[C@@H]([C@@H]([C@H]([C@H]([C@H]1C(=O)O)O)O)O)O
InChI
1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1
InChI key
AEMOLEFTQBMNLQ-BKBMJHBISA-N
General description
Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.
Application
Polygalacturonic acid has been used:
- in the preabsorption of 2F4 primary antibody solution
- as a substrate to determine polygalacturonase activity
- in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases
Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca2+ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.
Biochem/physiol Actions
In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.
Other Notes
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
Mouyong Zou et al.
PloS one, 8(11), e79357-e79357 (2013-11-16)
An alkaline polygalacturonate lyase (PGL) from Bacillus subtilis 7-3-3, PelC, with diverse depolymerization abilities for different pectin substrates was found. The PGL activity of PelC decreased with increasing degree of methyl esterification of the substrate. PelA and PelC displayed notable
Biotechnology for Livestock Production, 246-246 (1989)
L Kurillová et al.
Journal of microencapsulation, 17(3), 279-296 (2000-05-20)
The structure of standard and stabilized calcium pectate gel (CPG) beads has been examined by scanning (SEM) and transmission (TEM) electron microscopy. A two-stage crosslinking procedure with polyethyleneimine (PEI) and glutaraldehyde (GA) led to the formation of a more compact
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3889-25G | 04061834373193 |
| P3889-5G | 04061834373209 |
| P3889-500G | 04061832630335 |
| P3889-100G | 04061834373186 |