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P3326

Platelet-Derived Growth Factor-AB human

Name: Platelet-Derived Growth Factor-AB human
Brand: SIGMA

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-AB

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00213024

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE and N-terminal analysis)

Form:

lyophilized powder

Mol wt:

27-31 kDa

Impurities:

≤1 EU/μg

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Properties

Product Name

Platelet-Derived Growth Factor-AB human, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

10-100 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

27-31 kDa

packaging

pkg of 10 μg, pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P04085, P01127

storage temp.

−20°C

Gene Information

human ... PDGFA(5154), PDGFB(5155)

Description

General description

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

Application

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Biochem/physiol Actions

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

Physical form

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Other Notes

Heterodimer of the A and B chains of PDGF disulfide linked.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Articles

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Global Trade Item Number

SKU	GTIN
P3326-10UG	04061834367673
P3326-50UG	04061834367680