P2980
Pertussis toxin
from Bordetella pertussis, buffered aqueous glycerol solution, protein endotoxin
Synonym(s):
Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen
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About This Item
Form:
buffered aqueous glycerol solution
Quality level:
Product Name
Pertussis toxin from Bordetella pertussis, buffered aqueous glycerol solution
description
aseptically packaged
Quality Level
form
buffered aqueous glycerol solution
shipped in
dry ice
storage temp.
−20°C
SMILES string
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC
InChI
1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3
InChI key
WOPKHAQDUMDJIY-UHFFFAOYSA-N
Gene Information
Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)
General description
Pertussis (Pertussis) toxin (PTX) is a protein isolated from the bacterium Bordatella (Bordatella) coccobaccillus. The toxin is excreted in a non-active state. When the B oligomer of the bacterium, Bordatella coccobacillus, comes in contact with the cell membrane, an S1 subunit of its A protomer is activated. This activation may be mediated by glutathione and/or ATP.
Application
Inactive toxin form released from B. pertussis.
Pertussis toxin from Bordetella pertussis has been used: to enhance cardiac myosin immunization of rats to induce myocarditis; to break down the blood–brain barrier in the Cuprizone and cuprizone/MOG-EAE model; as an inhibitor of Gαi signaling
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.
Biochem/physiol Actions
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt.
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.
Features and Benefits
This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Physical form
Aseptically filled solution in 50% glycerol, containing 50 mM TRIS, 10 mM glycine, 0.5 M NaCl, pH 7.5
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Cyclic nucleotides like cAMP modulate cell function via PKA activation and ion channels.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P2980-.2MG | 04061838256140 |
| P2980-50UG | 04061838256157 |