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MilliporeSigma

P1644

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (20 °C)

Synonym(s):

Percoll Density Gradient Media

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About This Item

MDL number:
MFCD00131830
UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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sterility

aseptically filled

Quality Level

200

form

colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )

technique(s)

cell culture | mammalian: suitable

color

clear colorless to faint yellow

pH

8.5-9.5 (20 °C)

application(s)

hematology
histology

storage temp.

2-8°C

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General description

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials.

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used in cell separation and organelle isolation.
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll® has been used in sperm preparation to study the energy requirements of bovine embryos.

Legal Information

Percoll is a registered trademark of Cytiva

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCL4381
SLCK2057
SLCK2056
SLCK1189
SLCK0826

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Rajasree Sreedharan et al.
Kidney international, 86(3), 515-524 (2014-05-09)
Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral
T Hirano et al.
The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (approximately 3-micron long), which runs through a metaphase plate-like structure consisting
Guilherme D Melo et al.
Scientific reports, 7(1), 8454-8454 (2017-08-18)
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using
Tanja Zöller et al.
International journal of molecular sciences, 19(3) (2018-03-02)
Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we
R Hovius et al.
Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different
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Articles

Centrifugation Basics

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

Protocols

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

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