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P1526

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, solid

Synonym(s):

OPD

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
24898213
UNSPSC Code:
12352204
EC Number:
210-418-7
MDL number:
MFCD00012966
Beilstein/REAXYS Number:
3912045
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Product Name

o-Phenylenediamine dihydrochloride, peroxidase substrate

Quality Level

300

form

solid

color

white to off-white

mp

258 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.

Disclaimer

May darken in storage.

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Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
029K5304
088K5300
123K5310
116K5313
107H5361

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Articles

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Global Trade Item Number

SKUGTIN
P1526-10G04061834355571
P1526-50G04061834355618
P1526-100G04061833631225
P1526-1KG04061823207768
P1526-25G04061834355588