N3910
Neutralization Solution B
Synonym(s):
DNA neutralization solution, neutralization buffer DNA extraction
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161703
grade
Molecular Biology
Quality Level
form
liquid
feature
Direct PCR
technique(s)
DNA amplification: suitable, DNA extraction: suitable, PCR: suitable
input
crude DNA
storage temp.
−20°C
General description
This Neutralization Buffer B is intended for use with Sigma′s REDExtract-N-Amp™ Tissue PCR Kits. This buffer is used to neutralize the DNA extract and be combined with the REDExtract-N-Amp PCR Reaction Mix and PCR primers to amplify target DNA. The REDExtract-N-Amp Kits include a sufficient volume of Neutralization Buffer B for at least one neutralization per extraction. However, where additional amplifications are required, supplemental Neutralization buffer may be needed.
Application
Neutralization Solution B has been used in the modified Sigma Extract-N-Amp™ extraction procedure. It has also been used in genotyping of mice, for neutralizing the DNA extract prior to polymerase chain reaction (PCR) using Sigma′s REDExtract-N-Amp™ PCR Reaction Mix.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
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Protocols
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| N3910-2.5ML | 04061837301117 |
| N3910-240ML | 04061837301131 |
| N3910-6ML | 04061837301186 |
| N3910-1.2ML | 04061837301094 |
| N3910-24ML | 04061834116387 |
| N3910-60ML | 04061837301162 |