MAK137
Phospholipase D Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
PLD Activity Assay Kit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164)
mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908)
rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)
General description
Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.
Application
Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.
Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Biochem/physiol Actions
In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
Scott S A, et al.
Nature Chemical Biology, 5(2), 108-108 (2009)
Przemysław Bernat et al.
Journal of plant physiology, 229, 158-163 (2018-08-11)
2,4-dichlorophenoxyacetic acid (2,4-D) is among the most commonly used herbicides applied for weed control during wheat cultivation. However, its application could affect wheat growth. The present study investigates the effect of the ascomycetous fungus Trichoderma harzianum on lipid peroxidation, phospholipids
Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAK137-1KT | 04061834065524 |