MAK055
AST Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Aspartate Aminotransferase Activity Assay Kit, Glutamate-Oxaloacetate Transaminase Activity Assay Kit, SGOT Activity Assay Kit, Serum Glutamate-Oxaloacetate Transaminase Activity Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases; cardiovascular diseases
storage temp.
−20°C
Gene Information
human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)
Related Categories
General description
The new AST Assay Kit, MAK467, is now available! Aspartate Aminotransferase (AST), formerly called serum glutamate-oxaloacetate transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. Similar to Alanine Aminotransferase (ALT), AST levels in blood are commonly used as a marker for liver function. However, AST has a broader tissue distribution than ALT and perturbations in AST levels can occur in response to diseases or injuries in multiple tissues including skeletal and heart.
Application
AST Activity Assay Kit has been used to determine the activity of aspartate aminotransferase in samples.
Suitable for the measurement of aspartate aminotransferase (AST) activity in biological samples including tissue, cells and serum
Biochem/physiol Actions
The Aspartate Aminotransferase Activity Assay kit provides a simple and direct procedure for measuring AST activity in a variety of samples. In this kit, the transfer of an amino group from aspartate to α-ketoglutarate results in the generation of glutamate, resulting in the production of a colorimetric (450 nm) product proportional to the AST enzymatic activity present. One unit of AST is the amount of enzyme that will generate 1.0 μmole of glutamate per minute at pH 8.0 at 37 °C.
Features and Benefits
Compatible with high-throughput handling systems.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
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