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MilliporeSigma

M0325

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It is used for the cultivation of a wide variety of cells grown in monolayers. MEM includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. May be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Application

Minimum Essential Medium Eagle has been used as a culture medium for:
  • thyroid cancer cell lines
  • macrophage-like DH82 cells
  • vero CCL-81 cells



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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Sharanjeet Atwal et al.
mBio, 12(4), e0134221-e0134221 (2021-07-28)
Peptidoglycan (PG) is a highly cross-linked peptide-glycan mesh that confers structural rigidity and shape to most bacterial cells. Polymerization of new PG is usually achieved by the concerted activity of two membrane-bound machineries, class-A penicillin binding proteins (aPBPs) and class-B
Fang Feng et al.
Cancer research, 78(21), 6121-6133 (2018-09-16)
The sodium iodide symporter (NIS) is a classical iodide pump typically localized within the cell plasma membrane in thyroid cells, where NIS expression is believed to ensure success of mainstay radioiodide therapy in thyroid cancers. Although radioiodide uptake is generally
Jose Manuel Condor Capcha et al.
Frontiers in cardiovascular medicine, 7, 618651-618651 (2021-02-02)
The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus requires reliable assays for studying viral entry mechanisms which remains poorly understood. This knowledge is important for the development of therapeutic approaches to control SARS-CoV-2 infection by permitting the screening for neutralizing



Global Trade Item Number

SKUGTIN
M0325-500ML04061837847264