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MilliporeSigma

L9234

LB Broth (Lennox)

Liquid microbial growth medium

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About This Item

UNSPSC Code:
12352202
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grade

Molecular Biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

General description

Liquid media is also termed as broth. It has nutrients dissolved in water. Luria-Bertani broth (LB broth) is used in naphthalene degradation experiments.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
LB Broth has been used to culture P. aeruginosa.

Biochem/physiol Actions

LB Lennox media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Features and Benefits

LB Lennox media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Other Notes

10 g/L Tryptone
5.0 g/L Yeast Extract
5.0 g/L NaCl


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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocols

Initiating a Starter Culture


Pseudomonas toxin pyocyanin triggers autophagy: Implications for pathoadaptive mutations
Yang ZS, et al.
Autophagy, 12(6), 1015-1028 (2016)
Understanding Microbes: A Laboratory Textbook for Microbiology (1989)
Su-Yun Chang et al.
Water environment research : a research publication of the Water Environment Federation, 87(1), 61-67 (2015-01-30)
Luria-Bertani broth and acetone were usually used in naphthalene degradation experiments as nutrient and solvent. However, their effect on the degradation was seldom mentioned. In this work, we investigated the effect of LB, naphthalene concentration, and acetone on the degradation



Global Trade Item Number

SKUGTIN
L9234-1L04061833966518
L9234-500ML04061833966525