L8629
Lectin from Phaseolus vulgaris (red kidney bean)
Erythroagglutinin PHA-E
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Quality Level
form
salt-free, lyophilized powder
potency
<10 μg per mL agglutination activity (with erythryocytes)
composition
Protein, ≥80% biuret
storage temp.
2-8°C
Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Preparation Note
Purified by a modification of the method of Leavitt.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.
Tested for leucoagglutination.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
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Related Content
Tuo Yang et al.
Cell reports, 29(9), 2917-2928 (2019-11-28)
Increased tolerance of Plasmodium falciparum to front-line artemisinin antimalarials (ARTs) is associated with mutations in Kelch13 (K13), although the precise role of K13 remains unclear. Here, we show that K13 mutations result in decreased expression of this protein, while mislocalization
R D Leavitt et al.
The Journal of biological chemistry, 252(9), 2961-2966 (1977-05-10)
Affinity-purified phytohemagglutinin from red kidney bean resolves into five isolectins by SP-Sephadex ion exchange chromatography. Recoveries ranging from 30 to 130 mg of protein for each isolectin are easily achieved. The isolectins have similar amino acid compositions which differ only
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L8629-5MG | 04061833263709 |