I5896
Interleukin-7 human
≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
IL-7
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
≤0.500 ng/mL EC50
mol wt
25 kDa
packaging
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
solubility
water: soluble 0.005-0.010 mL
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL7(3574)
Related Categories
General description
The interleukin-7 human (IL-7) gene is mapped to human chromosome 8q21.13. It is a non-hematopoietic cell-derived cytokine. IL-7 is majorly produced by the fibroblastic stromal cells, dendritic cells, thymus epithelial cells and skin keratinocytes. Structurally, IL-7 takes up an up-up-down-down 4-helix bundle topology and has two crossover loops.
Biochem/physiol Actions
IL-7 helix A is crucial for its interaction with the interleukin-7 human receptor (IL-7Rα). It is reported to enhance immunity during viral infections and may be useful in cancer immunotherapy. Mutation in the IL-7 gene is implicated in CD4+ T-cell lymphopenia.
Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 stimulates the proliferation of CD4/CD8 cells. Human and mouse IL-7 have 60% amino acid sequence identity.
Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It was first isolated by Namen in 1988. IL-7 has a number of known activities. It supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 also stimulates the proliferation of CD4/CD8 cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4, and IL-6. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. Antibodies against IL-2 affect its activity, suggesting that it functions through IL-2 production. Human natural IL-7 is 25 kDa glycoprotein that has 6 cysteine residues which are important for biological activity. Human and mouse IL-7 have 60% amino acid sequence homology.
Lymphoid cell growth factor which affects pre-B, pro-B and early T cells; supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A; stimulates the proliferation of CD4/CD8 cells.
Physical form
Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid containing 250 μg bovine serum albumin.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Jianbao Gao et al.
International journal of molecular sciences, 16(5), 10267-10280 (2015-05-09)
Interleukin-7 (IL-7) is a non-hematopoietic cell-derived cytokine with a central role in the adaptive immune system. It promotes lymphocyte development in the thymus and maintains survival of naive and memory T cell homeostasis in the periphery. Moreover, it is important
Soichiro Saito et al.
Oncology reports, 16(5), 949-956 (2006-10-04)
Biliary tract cancers carry dismal prognoses. It is commonly understood that chromosomal aberrations in cancer cells have prognostic and therapeutic implications. However, in biliary tract cancers the genetic changes have not yet been sufficiently studied. The aim of this study
Edwin Leeansyah et al.
PLoS pathogens, 11(8), e1005072-e1005072 (2015-08-22)
Mucosa-associated invariant T (MAIT) cells represent a large innate-like evolutionarily conserved antimicrobial T-cell subset in humans. MAIT cells recognize microbial riboflavin metabolites from a range of microbes presented by MR1 molecules. MAIT cells are impaired in several chronic diseases including
Fathollah Kalantar et al.
Iranian journal of immunology : IJI, 11(1), 1-12 (2014-03-19)
Type 2 diabetes (T2D) is a chronic metabolic disorder in which beta-cells are destroyed. The islet amyloid polypeptide (IAPP) produced by beta-cells has been reported to influence beta-cell destruction. To evaluate if IAPP can act as an autoantigen and therefore
M Cecilia Albareda et al.
Journal of immunology (Baltimore, Md. : 1950), 194(8), 3883-3889 (2015-03-15)
We have previously demonstrated that immune responses in subjects with chronic Trypanosoma cruzi infection display features common to other persistent infections with signs of T cell exhaustion. Alterations in cytokine receptor signal transduction have emerged as one of the cell-intrinsic
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