HT501850
Formalin solution, neutral buffered, 10%
histological tissue fixative
Synonym(s):
10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal
biological source
synthetic
Quality Level
concentration
10% formalin (approx. 4% formaldehyde)
color
colorless
pH
6.90-7.10
density
1.080 g/cm3
application(s)
hematology
histology
storage temp.
room temp
SMILES string
[O-]#[C+H2]
InChI
1S/CH2O/c1-2/h1H2
InChI key
WSFSSNUMVMOOMR-UHFFFAOYSA-N
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General description
10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
Application
Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
- It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
- It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
- It is used for routine histological staining, providing clear and detailed images of cellular structures.
Popular general purpose histological fixative.
Biochem/physiol Actions
Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.
Features and Benefits
- 10% NBF is well-suited for large throughput laboratories.
- It requires a relatively short period of fixation.
- It can be used for the long-term storage of tissue.
Physical form
Neutral buffered solution
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
wgk_germany
WGK 3
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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