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MilliporeSigma

GE17-0120-01

Sepharose™ 4B

Cytiva 17-0120-01, pack of 1 L

Synonym(s):

agarose resin, chromatography resin, coupling matrix, cytiva 17-0120-01, filtration gel, unactivated resin

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About This Item

CAS Number:
9012-36-6
MDL number:
MFCD08457579
UNSPSC Code:
41106500
NACRES:
NA.56

Key Documents

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packaging

pack of 1 L

manufacturer/tradename

Cytiva 17-0120-01

matrix

4% agarose

particle size

45-165 μm

cleaning in place

4-9

working range

4-9

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Sepharose™ 4B is a well-proven Agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Application

Sepharose™ is a bead-formed Agarose-based gel filtration matrix. Sepharose™ is available with 3 different Agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight


Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents

Features and Benefits

  • 4% Agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

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The agarose migration inhibition technique for in vitro demonstration of cell-mediated immunity in man. A review.
J E Clausen
Danish medical bulletin, 22(5), 181-194 (1975-07-01)
Jana Wolf et al.
The EMBO journal, 33(14), 1514-1526 (2014-05-30)
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds
Sudhir Chandna et al.
International journal of radiation biology, 90(5), 401-406 (2014-02-18)
Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. Macrocolony formation
Yan Hong et al.
Journal of chromatography. A, 1342, 30-36 (2014-04-02)
Previously, we studied bovine serum albumin (BSA) uptake to poly(ethylenimine) (PEI)-grafted Sepharose resins, and an ionic capacity (IC) range (600-740mmol/L) for steep increases of both protein capacity (qm) and effective pore diffusion coefficient (De) was found. In this work, seven
Sieving by agarose gels and its use during pulsed-field electrophoresis.
P Serwer
Biotechnology & genetic engineering reviews, 8, 319-343 (1990-01-01)
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