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MilliporeSigma

G8507

α-Galactosidase from green coffee beans

ammonium sulfate suspension, ≥9 units/mg protein

Synonym(s):

alpha-Galactosidase, α-D-Galactoside galactohydrolase, Melibiase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥9 units/mg protein
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form

ammonium sulfate suspension

Quality Level

specific activity

≥9 units/mg protein

storage temp.

2-8°C

General description

α-Galactosidase from green coffee beans is encoded by the GLA gene.

Application

α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

Biochem/physiol Actions

α-Galactosidase from green coffee beans cleaves the terminal α-galactose from the oligosaccharides. α-Galactosidase deficiency is associated with Fabry disease.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 6.0, containing BSA

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per min at pH 6.5 at 25 °C.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Related Content


Pig xenogeneic antigen modification with green coffee bean alpha-galactosidase
Luo Y, et al.
Xenotransplantation, 6(4), 238-248 (1999)
A prophylactic alpha-Gal-based glycovaccine effectively protects against murine acute Chagas disease
Portillo S, etal.
NPJ vaccines, 4(1), 13-13 (2019)
Structure-function relationships in alpha-galactosidase A
Garman SC
Acta Paediatrica (Oslo, Norway : 1992), 96, 6-16 (2007)



Global Trade Item Number

SKUGTIN
G8507-25UN04061832844206
G8507-5UN04061833644058
G8507-50UN04061833644041