G5768
Gamborg′s B-5 Basal Salt Mixture
fine powder, suitable for plant cell culture
Synonym(s):
B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.72
form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Gamborg′ B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.
Application
Gamborg′s B-5 Basal Salt Mixture has been used:
- as a constituent during the preparation of TEX buffer
- as a media to maintain potato suspension and callus cultures
- as a media in the primary-node transformation method
Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Preparation Note
Formulated to contain 3.1 grams of powder per liter of medium.
With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Ashish Baldi et al.
Biotechnology letters, 30(9), 1671-1677 (2008-04-23)
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and
Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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