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MilliporeSigma

G5516

Sigma-Aldrich

Glycerol

greener alternative

Molecular Biology, ≥99.0%

Synonym(s):

1,2,3-Propanetriol, Glycerin

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About This Item

Linear Formula:
HOCH2CH(OH)CH2OH
CAS Number:
56-81-5
Molecular Weight:
92.09
Beilstein/REAXYS Number:
635685
EC Number:
200-289-5
MDL number:
MFCD00004722
UNSPSC Code:
50161901
PubChem Substance ID:
24895216
NACRES:
NA.31
Grade:
Molecular Biology
reagent grade
Assay:
≥99.0%
Technique(s):
MALDI-MS: suitable
cryopreservation: suitable
Application(s):
life science and biopharma
microbiology
sample preservation
Bp:
182 °C/20 mmHg (lit.)
Vapor pressure:
<1 mmHg ( 20 °C)

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grade

Molecular Biology
reagent grade

Quality Level

200

vapor density

3.1 (vs air)

vapor pressure

<1 mmHg ( 20 °C)

assay

≥99.0%

form

viscous liquid

autoignition temp.

698 °F

expl. lim.

≥2.7-19.0 %

greener alternative product characteristics

Safer Solvents and Auxiliaries
Design for Degradation
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

MALDI-MS: suitable
cryopreservation: suitable

impurities

<5 ppm Iron
<5 ppm heavy metal
<5 ppm magnesium

refractive index

n20/D 1.474 (lit.)

pH

5.5-8

bp

182 °C/20 mmHg (lit.)

mp

20 °C (lit.)

solubility

water: 1 mL/mL, clear, colorless

density

1.25 g/mL (lit.)

cation traces

Fe: ≤5 ppm
Mg: ≤5 ppm
heavy metals (as Pb): <5 ppm

application(s)

life science and biopharma
microbiology
sample preservation

foreign activity

DNase, RNase, NICKase, and protease, none detected

greener alternative category

Aligned

SMILES string

OCC(O)CO

InChI

1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2

InChI key

PEDCQBHIVMGVHV-UHFFFAOYSA-N

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General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of the 12 Principles of Green Chemistry. Glycerol, with its high boiling point, low flammability, and polarity, is a standout choice. As a non-toxic and biodegradable compound, it aligns perfectly with the principles of ′Safer Solvents and Auxiliaries′ and ′Design for Degradation′. Click here for more information.

Application

Glycerol has been used as:
  • a component of mounting medium for immunofluorescence
  • a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
  • a fuel during the designing of enzymatic biofuel cells.
  • a liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
  • a matrix for fast atom bombardment MS.
Glycerol may be employed as a liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Features and Benefits

This product has been analyzed for the presence of nucleases and proteases.

Other Notes

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For additional information on our range of Biochemicals, please complete this form.
The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

390.2 °F - Pensky-Martens closed cup

flash_point_c

199 °C - Pensky-Martens closed cup

ppe

Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
WXBD6788V
WXBD6802V
SHBN4019
SHBN4011
SHBN4006

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Nur Arifah Ismail et al.
Materials science & engineering. C, Materials for biological applications, 103, 109770-109770 (2019-07-28)
In this work, the potential of titanium dioxide nanoparticles incorporated gellan gum (GG + TiO2-NPs) biofilm as wound dressing material was investigated. The GG + TiO2-NPs biofilm was prepared via evaporative casting technique and was characterized using FTIR, XRD, and SEM to study their
Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium
Collins, L., and Scott G. Franzblau
Antimicrobial Agents and Chemotherapy, 41 (5), 1004-1009 (1997)
Development of glycerol/O 2 biofuel cell
Arechederra, Robert L., Becky L. Treu, and Shelley D. Minteer
Journal of Power Sources, 173 (1), 156-161 (2007)
The Future of Glycerol
Mario Pagliaro, Michele Rossi
Royal Society of Chemistry, 170-170 (2010)
Analysis of native milk oligosaccharides directly from thin-layer chromatography plates by matrix-assisted laser desorption/ionization orthogonal-time-of-flight mass spectrometry with a glycerol matrix
Dreisewerd, Klaus, et al
Journal of the American Society For Mass Spectrometry, 17(2), 139-150 (2006)
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Protocols

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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