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G5035

Granulocyte-Macrophage Colony-Stimulating Factor human

Name: Granulocyte-Macrophage Colony-Stimulating Factor human
Brand: SIGMA

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

GM-CSF human

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About This Item

MDL number:

MFCD00166611

NACRES:

NA.77

UNSPSC Code:

12352202

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥98% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

14.6 kDa

Impurities:

≤1.000 EU/μg

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Properties

Product Name

Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤0.1 ng/mL EC₅₀

mol wt

14.6 kDa

packaging

pkg of 4X5 μg, pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

solubility

water: soluble 0.010 mL, clear, colorless

UniProt accession no.

P04141

storage temp.

−20°C

Description

General description

Package size based on protein content

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
G5035-4X5UG	04061833632888
G5035-5UG	04061833632895