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MilliporeSigma

FG4815

Sigma-Aldrich

DMEM/F-12

With stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-alanyl-L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered
sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

phenol red: yes
stable glutamine: yes
glucose: high
HEPES: no
NaHCO3: yes
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture. This DMEM F-12 Ham medium differs from the original formula because it is supplemented with L-alanyl-L-glutamine dipeptide. This dipeptide provides a more stable form of glutamine for use in cell cultures as glutamine alone is highly unstable in cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture human fibroblasts.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL1634
RNBJ4043

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Articles

Nutrient Mixture F-12 Ham Formulation

Nutrient Mixture F-12 Ham Formulation

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