F8305
FRK (208-end), active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
GTK, PTK5, RAK
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
847-1147 nmol/min·mg
mol wt
~60 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... FRK(2444)
General description
FRK (fyn-related kinase) is a tyrosine kinase, with a molecular weight of 54kDa. It contains SH2 and SH3 (src homology) domains. It is predominantly expressed in tissues of epithelial origin such as, liver, kidney, breast and colon. Its SH2 domain contains a putative bipartite nuclear localization signal.
Biochem/physiol Actions
FRK (fyn-related kinase) controls growth by phosphorylating perinuclear proteins. It inhibits cell growth both in a pRb (retinoblastoma)-dependent and independent manner. It cause cell cycle arrest at G1 phase in breast cancer cells. Certain mutations in this gene lead to its constitutive activation, as seen in hepatocellular adenomas. Its up-regulation in gliomas prevents both invasiveness and migration of glioma cells. This is attributable to its promotion of N-cadherin/β-catenin complex formation.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Qiong Shi et al.
Journal of molecular neuroscience : MN, 55(1), 32-41 (2014-06-28)
Fyn-related kinase (FRK), a member of Src-related tyrosine kinases, is recently reported to function as a potent tumor suppressor in several cancer types. Our previous study has also shown that FRK over-expression inhibited the migration and invasion of glioma cells.
Tanya Meyer et al.
International journal of cancer, 104(2), 139-146 (2003-02-06)
Rak is a 54 kDa protein tyrosine kinase originally isolated from breast cancer cells and expressed in epithelial cells. It resembles the protooncogene Src structurally but lacks an amino-terminal myristylation site and localizes to the nuclear and perinuclear regions of
Camilla Pilati et al.
Cancer cell, 25(4), 428-441 (2014-04-17)
Hepatocellular adenomas (HCA) are benign liver tumors predominantly developed in women using oral contraceptives. Here, exome sequencing identified recurrent somatic FRK mutations that induce constitutive kinase activity, STAT3 activation, and cell proliferation sensitive to Src inhibitors. We also found uncommon
W G Cance et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 5(12), 1347-1355 (1994-12-01)
We have identified a new tyrosine kinase from human breast cancer cells called Rak (a Russian word for cancer) that shares 51% identity with c-Src. Sequencing of the full-length complementary DNA revealed that Rak is a tyrosine kinase with a
Michael Welsh et al.
The Biochemical journal, 382(Pt 1), 261-268 (2004-06-10)
Hallmarks of the inflammatory process in Type I diabetes are macrophage activation, local release of beta-cell-toxic cytokines and infiltration of cytotoxic T lymphocytes. We have observed recently that mice overexpressing active FRK (fyn-related kinase)/RAK (previously named GTK/Bsk/IYK, where GTK stands
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