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MilliporeSigma

F4648

Sigma-Aldrich

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

MDL number:
MFCD00132253
UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

Quality Level

200

form

tablet

solubility

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

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Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections
  • for staining chondrogenic spheroids in immunohistochemistry studies
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Preparation Note

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

hcodes

H314,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC3536
SLBT4170
SLBQ5628V
SLBM2372V
SLBH9987V

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Parsch D, et al.
Journal of Molecular Medicine, 82(1), 49-55 (2004)
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Yang Q, et al.
The AAPS Journal, 10(4), 614-614 (2008)
Plasticity of endothelial cells: rapid dedifferentiation of freshly isolated high endothelial venule endothelial cells outside the lymphoid tissue microenvironment
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