F1283
Fetal Bovine Serum
Fetal Bovine Serum, USA origin, Ultra Low IgG
Synonym(s):
FBS, FCS, Fetal Calf Serum
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About This Item
NACRES:
NA.71
UNSPSC Code:
12352207
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
biological source
bovine fetus
Quality Level
sterility
sterile-filtered
product line
FBS Select
composition
Bovine IgG, <5 μg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
storage condition
avoid repeated freeze/thaw cycles, protect from light
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
pH
6.8-8.1
osmolality
260-330 mOsm/kg H2O
shipped in
dry ice
storage temp.
−20°C
General description
Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Packaging
Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.
Preparation Note
The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Analysis Note
Endotoxin and hemoglobin tested
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F1283-500ML | 04061838095213 |
| F1283-100ML | 04061838069009 |