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ED4SS

Sigma-Aldrich

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate

99.0-102.0% (titration)

Synonym(s):

EDTA tetrasodium salt, Edathamil, Tetrasodium ethylenediaminetetraacetate dihydrate

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About This Item

Linear Formula:
(NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O
CAS Number:
10378-23-1
Molecular Weight:
416.20
Beilstein/REAXYS Number:
3861753
EC Number:
200-573-9
MDL number:
MFCD00150026
UNSPSC Code:
12352202
PubChem Substance ID:
24894711
NACRES:
NA.77

Key Documents

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Quality Level

300

assay

99.0-102.0% (titration)

form

crystalline

reaction suitability

reagent type: chelator

solubility

H2O: soluble, clear, colorless

SMILES string

O.O.[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.4Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;;2*1H2/q;4*+1;;/p-4

InChI key

KSYNLCYTMRMCGG-UHFFFAOYSA-J

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General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid and a metal chelating agent. It comprises four carboxylic acid and two amine groups with lone-pair electrons that can chelate calcium and several other metal ions. 

Application

Ethylenediaminetetraacetic acid tetrasodium salt dihydrate has been used:
  • in the decalcification of femurs for the histoprocessing and staining of osteochondral tissue
  • as a component of NH4Cl lysis buffer for erythrocyte lysis and bulk characterization
  • for the isolation of crypts from the connective tissues of mice

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors.
Ethylenediaminetetraacetic acid (EDTA) is used to treat patients poisoned with heavy metal ions. It functions as a chelator of the zinc ion in the active site of metalloproteases. EDTA inhibits other metal ion-dependent proteases such as calcium-dependent cysteine proteases. It might interfere with biological events which are metal ion-dependent. EDTA inhibits platelet aggregation and is the most preferred anticoagulant for platelet counts. It associates with other active agents to modulate microorganisms and biofilms, like citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, and other antiseptics. EDTA is used as an anticoagulant for hematological testing, as it preserves the cellular components and morphology of blood cells. 

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM3662
SLCK7432
SLCK1120
SLCJ0039
SLCG9817

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