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MilliporeSigma

D7291

Sigma-Aldrich

Deoxyribonuclease I RNase-free solution from bovine pancreas

Synonym(s):

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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About This Item

CAS Number:
9003-98-9
EC Number:
3.1.21.1 (BRENDA, IUBMB)
EC Number:
232-667-0
MDL number:
MFCD00130918
UNSPSC Code:
12352204
NACRES:
NA.53

Key Documents

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biological source

bovine pancreas

Quality Level

200

grade

Molecular Biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





mol wt

29.1 kDa

concentration

5—15 mg protein/mL

technique(s)

DNA extraction: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00639

application(s)

cell analysis

foreign activity

RNase, none detected

storage temp.

−20°C

Gene Information

cow ... DNASE1(282217)

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCG2097
SLCF3566
SLCB6995
SLCB0600
SLBX0879

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S Homburg et al.
The Journal of cell biology, 150(2), 293-307 (2000-07-26)
We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein
Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis
Ohyama K, et al.
Endocrine Journal, EJ15-0370 (2015)
A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties
TAIT GCL and HARRIS WJ
European Journal of Biochemistry, 75(2), 357-364 (1977)
Site-specific protein-DNA photocrosslinking. Analysis of bacterial transcription initiation complexes.
N Naryshkin et al.
Methods in molecular biology (Clifton, N.J.), 148, 337-361 (2001-05-19)
Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats
Ohyama K, et al.
Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)
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Protocols

Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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