D4293

3,3′-Diaminobenzidine

Name: 3,3′-Diaminobenzidine
Brand: SIGMA

SIGMAFAST^™, chromogenic, tablet

Synonym(s):

DAB tablets

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About This Item

CAS Number:

91-95-2

MDL number:

MFCD00007725

UNSPSC Code:

12352204

PubChem Substance ID:

24893856

NACRES:

NA.83

Key Documents

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Product Name

SIGMAFAST^™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

Quality Level

200

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Detection Substrates & Enzymes

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Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST^™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

SIGMAFAST^™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

pictograms

GHS08,GHS05,GHS07

signalword

Danger

hcodes

H315,H318,H335,H341,H350

pcodes

P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

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Receptor activator NFkappaB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas

Menezes R, et al.

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(3), 404-409 (2006)

Expression of pluripotent stem cell markers in the human fetal testis

Kerr CL, et al.

Stem Cells, 26(2), 412-421 (2008)

Interstitial cells of Cajal are present in human extrahepatic bile ducts

Ahmadi O, et al.

Journal of Gastroenterology and Hepatology, 25(2), 277-285 (2010)

Cell division rates decrease with age, providing a potential explanation for the age-dependent deceleration in cancer incidence.

Cristian Tomasetti et al.

Proceedings of the National Academy of Sciences of the United States of America, 116(41), 20482-20488 (2019-09-25)

A new evaluation of previously published data suggested to us that the accumulation of mutations might slow, rather than increase, as individuals age. To explain this unexpected finding, we hypothesized that normal stem cell division rates might decrease as we

Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis

Salakou S, et al.

In Vivo, 21(1), 123-132 (2007)

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3,3′-Diaminobenzidine