MDBK Cell Line bovine
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90050801, bovine kidney, Epithelial-like
Synonym(s):
MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
biological source
bovine kidney
packaging
tube of 5 μg 90050801-DNA-5UG
pkg of vial of cells 90050801-1VL
growth mode
Adherent
karyotype
Modal no. 51; hypodiploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Application
MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.
Biochem/physiol Actions
Bovine kidney
Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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