H9C2 (2-1) Cell Line from rat
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88092904, rat cardiovascular, Myoblast
Synonym(s):
H9c2 Cells
biological source
rat cardiovascular
packaging
tube of 5 μg 88092904-DNA-5UG
pkg of vial of cells 88092904-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Application
Differentiation studies
Biochem/physiol Actions
Rat BDIX heart myoblast
Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.
Other Notes
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