IMR-90 Cell Line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020204, human lung (fetal), Fibroblast
Synonym(s):
I90 Cells, IMR90 Cells, Institute for Medical Research-90 Cells
biological source
human lung (fetal)
description
Human Caucasian fetal lung fibroblast
packaging
tube of 5 μg 85020204-DNA-5UG
pkg of vial of cells 85020204-1VL
growth mode
Adherent
karyotype
2n = 46, diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Application
IMR-90 human cell line has been used to study the cytotoxicity of hydroxyapatite/gold/arginine (HAp/Au/arginine) nanoparticles.
Virus studies
Biochem/physiol Actions
Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.
Human Caucasian foetal lung fibroblast
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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