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MilliporeSigma

CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Synonym(s):

Caspase-3 Activity Fluorometric Kit

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About This Item

EC Number:
247-454-8
UNSPSC Code:
12161503
NACRES:
NA.84

Key Documents

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usage

sufficient for 1,000 multiwell tests
 sufficient for 100 standard tests (2 ml)

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Other Notes

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description
  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
128M4188V
11180421
046M4051V
11151219
054M4097V

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N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
Rebeca Vecino et al.
Scientific reports, 8(1), 1610-1610 (2018-01-27)
Brain preconditioning (PC) refers to a state of transient tolerance against a lethal insult that can be evoked by a prior mild event. It is thought that PC may induce different pathways responsible for neuroprotection, which may involve the attenuation
Ashish Kumar et al.
Scientific reports, 8(1), 2-2 (2018-01-10)
Recently, we have demonstrated that microRNA-31 (miR-31) overexpression is inherent to radiation-induced cell death in the highly radioresistant Sf9 insect cells, and regulates pro-apoptotic Bax translocation to mitochondria. In the present study, we report that at sub-lethal radiation doses for
Joana Silva et al.
BMC complementary and alternative medicine, 18(1), 58-58 (2018-02-16)
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. Although the causes of PD pathogenesis remain incomplete, some evidences has suggested that oxidative stress is an important mediator in its pathogenesis. The aim of this study
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