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MilliporeSigma

C6079

Sigma-Aldrich

Collagenase + protease inhibitor

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

Synonym(s):

Collagenase preparation, Protease inhibitor solution

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About This Item

EC Number:
3.4.24.3 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
Form:
solid
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

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biological source

bacterial (Clostridium histolyticum)

Quality Level

200

form

solid

specific activity

≥800 CDU/mg solid
2-5 FALGPA units/mg solid

mol wt

68-130 kDa

solubility

water: soluble

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... LOC296948(296948)

Related Categories

Application

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Biochem/physiol Actions

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca2+) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn2+ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Analysis Note

Also contains clostripain and non-specific neutral protease activities.

Other Notes

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
50150703
038M4111V
025M4027V
40140915
TEST

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Beverly Duncan et al.
PloS one, 5(7), e11427-e11427 (2010-07-14)
Double negative CD3(+)4(-)8(-) TCR alphabeta splenic cells (DNCD3) can suppress the immune responses to allo and xenografts, infectious agents, tumors, and some autoimmune disorders. However, little is known about their role in autoimmune diabetes, a disease characterized by the reduction
S P Gupta et al.
Bioorganic & medicinal chemistry, 11(14), 3065-3071 (2003-06-24)
A quantitative structure-activity relationship (QSAR) study has been made on eight different series of Clostridium histolyticum collegenase (ChC) inhibitors. These series are comprised of four different groups of sulfonylated amino acids and their corresponding hydroxamates. In each series, the inhibition
Association of immune system gene polymorphisms with quantitative features which are pathogenetically important in chronic viral hepatitis.
Goncharova, I., et al.
Molecular Biology, 42, 242-246 (2008)
C J Swanson et al.
Human immunology, 62(7), 739-749 (2001-06-26)
Recent progress in human islet transplantation demonstrates the feasibility of using purified human islets for treatment of type 1 diabetes mellitus; however, a shortage of human pancreata remains a major obstacle. This report describes methods to isolate porcine islets using
Brandon M Blobner et al.
American journal of physiology. Gastrointestinal and liver physiology, 321(5), G449-G460 (2021-09-16)
Chronic pancreatitis (CP) is a complex inflammatory disorder with numerous associated genetic and environmental risk factors. The most distressing characteristic of CP is recalcitrant pain, often requiring surgical resection including total pancreatectomy with islet autotransplantation (TPIAT). We studied five consented
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Protocols

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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