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MilliporeSigma

C0356

Sigma-Aldrich

Protein Extraction Reagent Type 4

Synonym(s):

Protein extraction solution

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About This Item

UNSPSC Code:
41116012
NACRES:
NA.56

Key Documents

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form

solid

Quality Level

200

solubility

deionized water: 13.6 g /15 ml at 25 °C, clear to hazy

General description

Protein Extraction Reagent Type 4, is a highly premixed, prequalified and effective protein solubilization and extraction reagent composed of urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Application

Protein extraction reagent type 4 is optimized for proteomic studies. Cellular and organelle membrane solubilizing reagent is ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. Reducing and alkylating reagents TBP (T7567) and iodoacetamide (A3221) are recommended for use with this reagent. Protein extraction reagent type 4 is also available as a component of the ProteoPrep® Sample.

Other Notes

Contains urea, thiourea, Trizma®, and detergent C7BzO (C0856).

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ8427
SLCF6389
SLBV1335
SLBT1871
SLBN1822V

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Meritxell Pérez-Hedo et al.
Proteome science, 10(1), 58-58 (2012-10-02)
Sesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter
John McCarthy et al.
Journal of proteome research, 2(3), 239-242 (2003-06-20)
Carbamylation is widely quoted as being a problem in 2-D gel analysis and the associated sample preparation steps. This modification occurs when iso-cyanate, a urea break-down product, covalently modifies lysine residues, thus inducing a change in isoelectric point. Urea is
Chawalit Kocharunchitt et al.
Molecular & cellular proteomics : MCP, 11(1), M111-M111 (2011-10-20)
An integrated transcriptomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass chilling in cold air. The response of
Ariadna Peremarti et al.
BMC genomics, 15, 125-125 (2014-02-14)
Leaf pigment content is an important trait involved in environmental interactions. In order to determine its impact on drought tolerance in wheat, we characterized a pale-green durum wheat mutant (Triticum turgidum L. var. durum) under contrasting water availability conditions. The
Gabriel A Grilo et al.
Journal of molecular and cellular cardiology, 139, 62-74 (2020-01-25)
Age-related remodeling of the heart causes structural and functional changes in the left ventricle (LV) that are associated with a high index of morbidities and mortality worldwide. Some cardiac pathologies in the elderly population vary between genders revealing that cardiac
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