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A2383

Sigma-Aldrich

Adenosine 5′-triphosphate disodium salt hydrate

≥99%, microbial, powder

Synonym(s):

ATP disodium salt

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About This Item

Empirical Formula (Hill Notation):
C10H14N5Na2O13P3 · xH2O
CAS Number:
34369-07-8
Molecular Weight:
551.14 (anhydrous basis)
EC Number:
213-579-1
MDL number:
MFCD00150755
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
24890481
NACRES:
NA.51

Key Documents

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Product Name

Adenosine 5′-triphosphate disodium salt hydrate, Grade I, ≥99%, from microbial

biological source

microbial

Quality Level

200

type

Grade I

assay

≥99%

form

powder

solubility

H2O: 50 mg/mL

storage temp.

−20°C

SMILES string

O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1

InChI key

NTBQNWBHIXNPRU-MSQVLRTGSA-L

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Application

Adenosine 5′-triphosphate disodium salt hydrate has been used:
  • to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures
  • bone marrow-derived macrophages (BMDM) treatment
  • as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)

Biochem/physiol Actions

Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo. ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP). ATP provides the metabolic energy to drive metabolic pumps. It serves as a coenzyme in a wide array of enzymatic reactions.
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000297297
0000297292
0000277012
0000282242
0000288576

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Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
Marta De Donato et al.
Scientific reports, 8(1), 16047-16047 (2018-10-31)
The NIMA (never in mitosis, gene A)-related kinase-6 (NEK6), which is implicated in cell cycle control and plays significant roles in tumorigenesis, is an attractive target for the development of novel anti-cancer drugs. Here we describe the discovery of a
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Protocols

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

Enzymatic Assay & ATP Sensitivity Test of Luciferase

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

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