Skip to Content
MilliporeSigma

A2237

Phosphatase, Alkaline shrimp

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonym(s):

Phosphatase, Alkaline from shrimp, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Recombinant:
expressed in proprietary host
Concentration:
≥900 DEA units/mL
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


recombinant

expressed in proprietary host

Quality Level

form

buffered aqueous glycerol solution

concentration

≥900 DEA units/mL

foreign activity

RNAse, DNAse, none detected

shipped in

dry ice

storage temp.

−20°C

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Naomi Ofer et al.
The Journal of biological chemistry, 288(16), 11287-11293 (2013-03-09)
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)



Global Trade Item Number

SKUGTIN
A2237-1KU04061833349878
A2237-500UN04061832754154